基于微卫星分子标记的浙江省不同滞育型迷卡斗蟋的遗传分化分析

【目的】迷卡斗蟋Velarifictorus micado存在两种显著不同的滞育型，即卵滞育和若虫滞育，而在长江流域附近两种滞育型种群存在广泛同域分布。本研究旨在探究浙江省同域分布不同滞育型迷卡斗蟋种群之间的遗传分化的情况。【方法】以浙江省百山祖、大盘山和天童山3个地区的不同滞育型的迷卡斗蟋6个种群共134个个体为研究材料，筛选具较高多态性的微卫星位点，并使用筛选获得的微卫星位点对这6个种群共134个个体进行种群遗传学分析。【结果】筛选出11对具较高多态性的微卫星位点；全部种群的平均等位基因数为13.803，平均观测杂合度为0.743，其中百山祖卵滞育种群观测杂合度最低，与其他种群间遗传分化最大。百山祖卵滞育种群与其他种群之间的基因交流程度很低，其余种群间均显示出高水平的基因流。【结论】浙江迷卡斗蟋不同地理种群及不同滞育型特征种群间遗传分化程度均较低，存在较频繁的基因交流，尚未表现出物种分化的趋势。

Analysis of the genetic differentiation ofVelarifictorus micado(Orthoptera: Gryllidae)with different diapause types in Zhejiang Province of eastern China based on microsatellitemarkers

【Aim】 Velarifictorus micado has two types of diapause, egg diapause and nymphaldiapause, which are sympatrically distributed in the Yangtze River basin. The objective ofthis study is to explore the genetic differentiation of V. micado populations betweendifferent diapause types sympatrically distributed in Zhejiang Province of eastern China.【Methods】Microsatellite loci with high polymorphism were screened with materials of 134individuals of six populations of V. micado with different diapause types from three areas,Baishanzu, Dapanshan and Tiantongshan, in Zhejiang Province, and the population geneticanalysis of the 134 individuals of six populations of V. micado was performed using thescreened microsatellite loci.【Results】 Eleven microsatellite loci with high polymorphismwere obtained. For all populations, the mean number of alleles was 13.803, and the meanobserved heterozygosity was 0.743. The observed heterozygosity of egg diapause populationsfrom Baishanzu was the lowest, indicating the largest genetic differentiation with otherpopulations. Besides, the gene flow between egg diapause populations from Baishanzu and theother populations was low, while the other populations showed higher levels of gene flow.【Conclusion】 There is low degree of genetic differentiation and high level of gene flowamong different populations of V. micado with different diapause types in Zhejiang, showingno trend of species differentiation.