Nucleotide sequence of the yeast glutathione S-transferase cDNA |
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| Authors: | H Tamaki H Kumagai T Tochikura |
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| Institution: | Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan. |
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| Abstract: | The nucleotide sequence (658 bp) of the cDNA coding for glutathione S-transferase Y-2 of yeast Issatchenkia orientalis was obtained. The cDNA clone contains an open reading frame of 570 nucleotides encoding a polypeptide comprising 190 amino acids with a molecular weight of 21,520. The primary amino acid sequence of the enzyme exhibits only 25.0% and 21.1% identity with 177 and 151 amino acid residues of maize glutathione S-transferase I and rat glutathione S-transferase Yb2, respectively. |
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