HPV检测早期筛查宫颈癌的临床意义——附妇科门诊2761例宫颈拭子HPV分析

目的探讨女性HPV DNA检测在宫颈癌防治方面的意义。方法应用DNA杂交技术对2 761例妇科门诊就诊者基因分型检测。结果 2 761例样本中,HPV感染有768例,阳性率27.82%,HPV感染人次972人次。检测高危型HPV（16,18,31,33,35,39,45,51,52,56,58,59,68）813人次,占感染总人次的83.64%;检出低危型HPV（6,11,42,43,44）73人次,占感染总人次7.51%;中国人群常见型HPV（53,66,CP8304）86人次,占感染总人次的8.85%。165例样本中包含了25种亚型的感染。结论 DNA杂交技术检测HPV基因分型,可一次检测多种亚型,有利于对HPV多重感染的诊断和宫颈癌的防治,可作为宫颈癌筛查的手段。

Application of human papillomavirus testing for early cervical cancer screening

Objective To investigate the distribution of human papillomavirus（HPV） genotype infections and explore the application of HPV DNA test in the prevention and treatment of cervix cancer.Method Totally 2 761 women were detected for HPV genotyping by using DNA hybridization technique.Result Of these 2 761 women,768 women（27.82%） were found to be HPV-positive.Among 972 cases of HPV infection 813（83.64%） were infected with high-risk HPV types（HPV-16,18,31,33,35,39,45,51,52,56,58,59,and 68）,73（7.51%） were with low-risk types（HPV-6,11,42,43,and 44）,and 86（8.85%） were with some common types（HPV-53,66,and P8304） among Chinese women.2～5 subtypes were detected in 165 patients.Conclusion DNA hybridization technique is useful for the diagnosis of multi-HPV infection and therefore for the prevention and treatment of cervical cancer.