Recombinant protein composed of Pseudomonas exotoxin A, outer membrane proteins I and F as vaccine against P. aeruginosa infection |
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| Authors: | T-Y Chen H-F Shang T-L Chen C-P Lin C-F Hui J Hwang |
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| Institution: | (1) Institute of Genetics, School of Life Sciences, National Yang-Ming University, Taipei, 115, Taiwan, ROC, TW;(2) Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 115, Taiwan, ROC e-mail: JH@ccvax.sinica.edu.tw Tel.: 8862-2789-9217 Fax: 8862-2782-6085, TW;(3) Institute of Zoology, Academia Sinica, Nankang, Taipei, 115, Taiwan, ROC, TW;(4) Department of Microbiology, Taipei Medical College, Taipei, Taiwan, TW;(5) Division of Drug Biology, National Laboratories of Foods and Drugs, Department of Health, Taipei, Taiwan, ROC, TW |
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| Abstract: | We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PEIF. The potential of PEIF as a vaccine
against Pseudomonas infection was evaluated in BALB/c mice and New Zealand white rabbits. We examined titers of anti-PE and anti-OprF antibodies,
and the ability both to neutralize PE cytotoxicity and to increase opsonophagocytic uptake of Pseudomonas aeruginosa strain PAO1, serogroups 2 and 6. The results showed that PEIF can induce antibodies not only to neutralize the PE cytotoxicity
but also to promote the uptake of various strains of P. aeruginosa by murine peritoneal macrophages. In a burned mouse model, PEIF afforded significant protection against infection by the
homologous P. aeruginosa strain PAO1, heterologous serogroup 2, and the PE hyperproducing strain PA103. These observations thus indicate that PEIF
may be used as a novel vaccine against P. aeruginosa infection.
Received: 10 March 1999 / Received revision: 21 April 1999 / Accepted: 16 May 1999 |
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