Identification of a membrane proteomic signature for human embryonic stem cells independent of culture conditions |
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Authors: | Linda Harkness Helle Christiansen Jan Nehlin Torben Barington Jens S Andersen Moustapha Kassem |
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Institution: | 1. Department of Endocrinology and Metabolism, Medical Biotechnology Center, University of Southern Denmark, Winsløwparken 25, 5000 Odense C, Denmark;2. Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark;3. Department of Clinical Immunology, Odense University Hospital, University of Southern Denmark, Odense 5000, Denmark;4. Center for Stem Cell Treatment, Odense University Hospital, University of Southern Denmark, Odense 5000, Denmark |
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Abstract: | Proteomic profiling of human embryonic stem cells (hESC) can identify cell fate determination and self-renewal biomarkers. Employing Fourier transform LC-ESI-MS/MS and MS3 mass spectrometry, we obtained a membrane proteomic signature overlapping between hESC cultured on mouse embryonic fibroblast (MEF) feeders and those grown under MEF-free culture conditions. We identified 444 transmembrane or membrane-associated proteins, of which 157 were common between both culture conditions. Functional annotation revealed CD antigens (10%), adhesion proteins (4%), proliferation-associated proteins (4%), receptors (41%), transport proteins (21%), structural proteins (5%), and proteins with miscellaneous functions (15%). In addition, 15 CD antigens and a number of surface marker molecules not previously observed in hESC at a proteome level, e.g., Nodal modulator 1, CD222, transgelin-2, and CD81, were identified. In conclusion, we describe the first membrane proteome profile of hESC that is independent of culture conditions. These data can be used to define the phenotype of hESC. |
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