Characterization of sugar transport in 2-deoxy-d-glucose resistant mutants of yeast |
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| Authors: | Srdjan Novak Tony D'Amore Inge Russell Graham G Stewart |
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| Institution: | (1) Research Department, Labatt Brewing Company Limited, 150 Simcoe Street, N6A 4M3 London, Ontario, Canada;(2) Present address: Faculty for Food Technology and Biotechnology, University of Zagreb, Zagreb, Yugoslavia |
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| Abstract: | Summary A number of 2-deoxy-d-glucose (2-DOG) resistant mutants exhibiting resistance to glucose repression were isolated from variousSaccharomyces yeast strains. Most of the mutants isolated were observed to have improved maltose uptake ability in the presence of glucose. Fermentation studies indicated that maltose was taken up at a faster rate and glucose taken up at a slower rate in the mutant strains compared to the parental strains, when these sugars were fermented together. When these sugars were fermented separately, only the 2-DOG resistant mutant obtained fromSaccharomyces cerevisiae strain 1190 exhibited alterations in glucose and maltose uptake compared to the parental strain. Kinetic analysis of sugar transport employing radiolabelled glucose and maltose indicated that both glucose and maltose were transported with higher rates in the mutant strain. These results suggested that the high affinity glucose transport system was regulated by glucose repression in the parental strain but was derepressed in the mutant. |
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