A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains |
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| Authors: | Estill S J Garcia J A |
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| Affiliation: | Department of Biochemistry and Division of Molecular Cardiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8573, USA. |
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| Abstract: | A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C57BL/6J:129S6/SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research. |
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