Response of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> roots to lipo-chitooligosaccharide from <Emphasis Type="Italic">Bradyrhizobium japonicum</Emphasis> and other chitin-like compounds |
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Authors: | Wajahatullah Khan Carlos Costa Alfred Souleimanov Balakrishnan Prithiviraj Donald L Smith |
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Institution: | (1) Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia;(2) University of Passo Fundo, Passo Fundo, RS, 99001-970, Brazil;(3) Department of Plant Science, Macdonald Campus of McGill University, 21111 Lakeshore, Ste., Anne-de-Bellevue, QC, H9X 3V9, Canada;(4) Department of Environmental Sciences, Nova Scotia Agricultural College, Truro, NS, B2N 5E3, Canada; |
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Abstract: | Lipo-chitooligosaccharides (LCOs) are bacteria-to-plant signals required for the establishment of rhizobia–legume nitrogen
fixing symbioses. The ability of LCO Nod Bj V (C18:1, MeFuc)] isolated from B. japonicum (strain 532C), and of oligomers of chitosan (tetramer, pentamer) and chitin (pentamer) to affect the developmental morphology
of roots in Arabidopsis thaliana (L.) Heynh ecotype Columbia (Col-0) was assessed using an interactive scanner-based image analysis system. LCOs have been
shown to play a role in plant organogenesis at nanomolar concentrations. LCO and the chitin pentamer promoted root growth
and development in Arabidopsis at concentrations of 10 nM and 100 μM, respectively. The LCO treated Arabidopsis plants had about 35% longer roots than untreated control plants. Similarly, treatment with 100 μM chitin pentamer (CHIT5)
resulted in 26% longer roots than the untreated plants; however, chitosan oligomer (CH4 or CH5) treated plants did not differ
from the control plants at either concentration (100 or 1 μM). Both LCOs and the chitin pentamer at higher concentrations
increased root surface area, mean root diameter and number of root tips. However, leaf area increase was observed only in
plants treated with LCO at 10 nM. |
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