Variability of Leishmania (Leishmania) infantum among stocks from immunocompromised, immunocompetent patients and dogs in Spain |
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| Authors: | Maribel Jiménez Montserrat Ferrer-Dufol Carmen Cañavate Beatriz Gutiérrez-Solar Ricardo Molina Fernando Lagun Rogelio López-Vélez Emilia Cercenado Esteban Daudén Julio Blazquez Conception Ladrón de Guevara Javier Gómez Julian de la Torre Carlos Barros Jordi Altes Teresa Serra Jorge Alvar |
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| Institution: | Laboratorio de Referenda de Leishmaniasis, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain; Unidad de Parasitología y Enfermedades Parasitarias, Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain; Centro de Investigations Clínicas, Instituto de Salud Carlos III, Madrid, Spain; Hospital Ramón y Cajal, Madrid, Spain; Hospital Universitario Gregorio Marañón, Madrid, Spain; Hospital de Princesa, Madrìd, Spain; Hospital General de Elche, Alicante, Spain; Hospital 'La Paz', Madrid, Spain; Hospital Severo Ochoa, Madrid, Spain; Hospital Universitario Reina Sofía, Córdoba, Spain; Complejo Hospitalario Móstoles-Alcorcón, Madrid, Spain; Hospital Juan March, Palma de Mallorca, Spain; Hospital de Son Dureta, Palma de Mallorca, Spain |
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| Abstract: | Abstract Leishmania (Leishmania) infantum is the causative agent of both the cutaneous and visceral forms of leishmaniasis in southwest Europe; the dog is the main reservoir. In order to identify the L. (L.) infantum zymodemes present in Spain, a total number of 85 Leishmania stocks isolated from dogs (31), HIV-positive patients (46) with visceral or cutaneous leishmaniasis, a patient with visceral leishmaniasis complicating renal transplantation (1) and immunocompetent patients (7) with visceral or cutaneous leishmaniasis, have been characterized by isoenzyme typing. All canine stocks were MON-1, which is the most widespread zymodeme in the Mediterranean area. In immunocompetent patients three zymodemes were found: MON-1 (2), MON-24 (2) and MON-34 (3). Nine different zymodemes were obtained in stocks from HTV co-infected patients, indicating a higher variability of L. (L.) infantum amongst them: MON-1 (in 21 stocks), MON-24 (7), MON-28 (1), MON-29 (3), MON-33 (7), MON-34 (1) and MON-183 (4). Two new zymodemes, MON-198 (1) and MON-199 (1), were described among HIV patients from Spain. The stock from the renal transplanted patient was MON-1. The exclusive presence of certain zymodemes in immunocompromised patients and their absence in typical cases of cutaneous and visceral |
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