| 球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究 |
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| 引用本文： | 张洁,蔡敬民,吴克,金胜先,潘仁瑞,樊美珍. 球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究[J]. 生态学杂志, 2004, 0(5): 863-866 |
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| 作者姓名： | 张洁 蔡敬民 吴克 金胜先 潘仁瑞 樊美珍 |
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| 作者单位： | 1. 合肥学院生物科学与技术系, 合肥 230022;2. 安徽农业大学虫生真菌研究所, 合肥 230036 |
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| 基金项目： | 安徽省教育厅自然科学基金项目(2000jl267);合肥学院科研基金项目;安徽省优秀青年科技基金资助项目(04043051) |
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| 摘 要： | 对球孢白僵菌(Beauveria bassiana)Bb174产几丁质酶进行了固态发酵条件及酶学特征的研究.结果表明,以4:1麸皮:蚕蛹粉、蛋白胨1g·L-1作为产酶最适培养基,在75g培养基中接种3ml液态种子,自然pH下28℃培养2d,酶活可达最高,为126U·g-1(干培养基).粗酶液的最适反应温度为40℃,最适反应pH5.0,在30～70℃保温1h,得半失活温度48℃.在30～40℃、pH4～6范围内,酶的性质最稳定.根据Lineweaver-Burk作图法,得到该酶的动力学参数Km为0.52mg·ml-1,Vm为0.7△E680·h-1.
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| 关 键 词： | 球孢白僵菌 几丁质酶 固态发酵 酶动力学 |
| 收稿时间： | 2002-11-12 |
Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation |
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| ZHANG Jie,CAI Jingmin,WU Ke,JIN Shengxian,PAN Renrui,FAN Meizhen. Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation[J]. Chinese Journal of Ecology, 2004, 0(5): 863-866 |
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| Authors: | ZHANG Jie CAI Jingmin WU Ke JIN Shengxian PAN Renrui FAN Meizhen |
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| Affiliation: | 1. Department of Biology Science and Technology, Hefei College, Hefei 230022, China;2. Institute of Economic Insects and Fungi, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | This paper studied the chitinase production of Beauveria bassiana Bb174 under solid state fermentation condition.The optimal medium consisted of wheat bran and silkworm chrysalis at the ratio of 4:1,supplemented with 1 g peptone·L-1 as nitrogen source and some other mineral nutrients.The enzyme activity reached 126 units per gram dry medium after cultured for 2 days at 28 ℃ and natural pH by inoculated 3 ml spore suspension into this medium.The optimal temperature and pH for chintinase production were 40 ℃ and 5.0,respectively.The temperature to lose 50% activity of the enzyme was 48 ℃ after incubated at 30～70 ℃ for 1 h.The enzyme was stable at 30～40 ℃ and pH 4～6,and the Km and Vmax values were 0.52 mg·ml-1 and 0.7△E680·h-1,respectively. |
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| Keywords: | Beauveria bassiana Chitinase Solid state fermentation Kinetic of enzyme reaction |
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