Ecotype-specific and chromosome-specific expansion of variant centromeric satellites in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
| |
Authors: | Hidetaka Ito Asuka Miura Kazuya Takashima Tetsuji Kakutani |
| |
Institution: | (1) Department of Integrated Genetics, National Institute of Genetics, Mishima Shizuoka, 411-8540, Japan |
| |
Abstract: | Despite the conserved roles and conserved protein machineries of centromeres, their nucleotide sequences can be highly diverse
even among related species. The diversity reflects rapid evolution, but the underlying mechanism is largely unknown. One approach
to monitor rapid evolution is examination of intra-specific variation. Here we report variant centromeric satellites of Arabidopsis thaliana found through survey of 103 natural accessions (ecotypes). Among them, a cluster of variant centromeric satellites was detected
in one ecotype, Cape Verde Islands (Cvi). Recombinant inbred mapping revealed that the variant satellites are distributed
in centromeric region of the chromosome 5 (CEN5) of this ecotype. This apparently recent variant accumulation is associated
with large deletion of a pericentromeric region and the expansion of satellite region. The variant satellite was bound to
HTR12 (centromeric variant histone H3), although expansion of the satellite was not associated with comparable increase in
the HTR12 binding. The results suggest that variant satellites with centromere function can rapidly accumulate in one centromere,
supporting the model that the satellite repeats in the array are homogenized by occasional unequal crossing-over, which has
a potential to generate an expansion of local sequence variants within a centromere cluster.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. |
| |
Keywords: | Repeat Evolution Centromeric histone H3 |
本文献已被 PubMed SpringerLink 等数据库收录! |
|