葡萄籽中原花青素对实验动物主动脉收缩和血小板聚集的影响

目的:研究葡萄籽中原花青素(PA)对大鼠离体主动脉平滑肌收缩活动和兔血小板聚集的影响.方法:采用大鼠离体主动脉环灌流方法,记录主动脉环张力变化,观察PA对去甲肾上腺素(NA)和KCl预收缩大鼠离体主动脉平滑肌收缩反应的舒张作用以及对NA量效曲线的影响.比浊法测定兔血小板聚集.结果:PA能明显抑制NA(10-6mol/L)预收缩大鼠离体主动脉环的反应,使NA量效曲线压低,最大反应降低,此作用无内皮依赖性,但对KCl预收缩主动脉环的舒张作用无明显影响,也不影响花生四烯酸(AA),ADP和胶原(collagen)蛋白诱导的兔血小板聚集.结论:PA能对抗NA而不影响KCl诱导的大鼠离体主动脉平滑肌的收缩,不影响兔血小板聚集.

Effects of proanthocyanidins on contractile activity of aortic smooth muscle and platelet aggregation in experimental animals

Aim: To study effects of proanthocyanidins(PA) on contractile activity of isolated aortic smooth muscle in rats and rabbit platelet aggregation.Methods: Isolated rat aortic muscle rings were adopted to observe the effects of PA on their contraction induced by noradrenaline(NA) or KCl,and their tensions were recorded by BL-310 experimental system of biological function. Rabbit platelet aggregation induced by arachidonic acid(AA),adenosine diphosphate(ADP),and collagen(Coll) was assayed by turbidimetry.Results: PA could significantly inhibit the contraction induced by NA(10~(-6) mol/L),low the concentration-response curves of NA and the maximal response on the endothelium-intact or endothelium-denuded aortic rings.But PA couldn't relax the aortic rings precontracted with KCl and had no influence on rabbit platelet aggregation induced by AA,ADP,and Coll.Conclusion: PA can inhibit the contraction induced by NA but not by KCl on isolated rat aortic rings.It also has no influence on rabbit platelet aggregation.