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花生种子贮藏蛋白的氨基酸组成分析及发育过程中17.5 kDa多肽的合成规律
引用本文:芦春斌,黄上志,汤学军,傅家瑞.花生种子贮藏蛋白的氨基酸组成分析及发育过程中17.5 kDa多肽的合成规律[J].热带亚热带植物学报,2000,8(4):339-345.
作者姓名:芦春斌  黄上志  汤学军  傅家瑞
作者单位:1. 暨南大学生殖免疫中心,广东,广州,510632
2. 中山大学生物科学与技术系,广东,广州,510275
基金项目:广东省自然科学基金(950090)资助项目
摘    要:分析了两种不同蛋白质组成类型花生的子叶总蛋白3个主要组分及高甲硫氨酸类型花生的60.5、41、38.5、18和17.5kDa多肽的氨基酸组成,结果表明它们均含有17种氨基酸,其中天冬氨酸、谷氨酸和精氨酸含量最高,而甲硫氨酸含量和半胱氨酸水平都极低。高甲硫氨酸类型品种的各组分的甲硫氨酸含量均显著高于低甲硫氨酸类型品种的对应组分的甲硫氨酸含量,在这两种类型花生中伴花生球蛋白Ⅱ都是甲硫氨酸含量最高的组分

关 键 词:花生种子  氨基酸组成  17.5kDa多肽  合成规律
文章编号:1005-3395(2000)04-0339-07
修稿时间:1999年11月12

COMPOSITION OF AMINO ACID IN STORAGE PROTEINS AND CHANGES IN 17.5 kDa POLYPEPTIDE SYNTHESIS DURING PEANUT SEED DEVELOPMENT
LU Chun-bin,HUANG Shang-zhi,TANG Xuejun,FU Jia-rui.COMPOSITION OF AMINO ACID IN STORAGE PROTEINS AND CHANGES IN 17.5 kDa POLYPEPTIDE SYNTHESIS DURING PEANUT SEED DEVELOPMENT[J].Journal of Tropical and Subtropical Botany,2000,8(4):339-345.
Authors:LU Chun-bin  HUANG Shang-zhi  TANG Xuejun  FU Jia-rui
Abstract:Three major components of salt-soluble proteins in peanut seeds were roughly purified on Sephadex G-100 gel filtration. Five purified polypeptides, 60.5, 41, 38.5, 18 and 17.5 kDa polypeptides, were harvested with high purity and productivity. Amino acid composition of the three major components (arachin, conarachin I and conarachin II) and the five purified polypeptides were analyzed. It was shown that 17 amino acids, including 7 essential amino acids, were detectable. The contents of asparatic acid, glutamic acid and arginine were high, whereas methionine and cysteine were extremely low. The methionine levels of three major components in methionine-rich cultivar Shanyou 523 were obviously higher than those in cultivar Haihua 1. The methionine level of conarachin II in three major components was the highest in the two cultivars. Results showed that highest methionine in 17.5 kDa polypeptide was observed in the five purified polypeptides. Western blot showed synthetic changes in 17.5 kDa polypeptide during development of peanut seeds.
Keywords:Peanut seed  Amino acid composition  17  5 kDa polypeptide
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