Adriamycin induced myocardial failure in rats: Protective role of Centella asiatica |
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Authors: | A Gnanapragasam S Yogeeta R Subhashini K K Ebenezar V Sathish Dr T Devaki |
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Institution: | (1) Department of Biochemistry, University of Madras, Guindy Campus, Chennai, 600 025, Tamilnadu, India;(2) Department of Physiology and Biophysics, University of Calgary, Calgary, Canada;(3) Department of Physiology and pharmacology, University of Western Ontario, London, Canada |
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Abstract: | Generation of reactive oxygen species and mitochondrial dysfunction has been implicated in adriamycin induced cardiotoxicity.
Mitochondrial dysfunction is characterized by the accumulation of oxidized lipids, proteins and DNA, leading to disorganization
of mitochondrial structure and systolic failure. The present study was aimed to evaluate the efficacy of Centella asiatica on the mitochondrial enzymes; mitochondrial antioxidant status in adriamycin induced myocardial injury. Adriamycin (2.5 mg/kg
body wt., i.p.) induced mitochondrial damage in rats was assessed in terms of decreased activities (p< 0.05) of cardiac marker enzymes (lactate dehydrogenase, creatine phosphokinase, amino transferases), TCA cycle enzymes (isocitrate
dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase, respiratory marker enzymes (NADH-dehydrogenase, cytochrome-C-oxidase),
mitochondrial antioxidant enzymes (GPx, GSH, SOD,CAT) and increased (p< 0.05) level of lipid peroxidation. Mitochondrial damage was confirmed by transmission electron microscopic examination.
Pre-co-treatment with aqueous extract of Centella asiatica (200 mg/kg body wt, oral) effectively counteracted the alterations in mitochondrial enzymes and mitochondrial defense system.
In addition, transmission electron microscopy study confirms the restoration of cellular normalcy and accredits the cytoprotective
role of Centella asiatica against adriamycin induced myocardial injury. Our results demonstrated elevated oxidative stress and mitochondrial dysfunction
in adriamycin treated rats. Moreover, on the basis of our findings it may be concluded that the aqueous extract of C. asiatica not only possesses antioxidant properties but it may also reduce the extent of mitochondrial damage |
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Keywords: | Adriamycin Centella asiatica Myocardial injury Lipid peroxidation Mitochondrial enzymes Antioxidants |
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