Role of hydroperoxide lyase in white-backed planthopper (Sogatella furcifera Horváth)-induced resistance to bacterial blight in rice, Oryza sativa L. |
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Authors: | Kenji Gomi Masaru Satoh Rika Ozawa Yumi Shinonaga Sachiyo Sanada Katsutomo Sasaki Masaya Matsumura Yuko Ohashi Hiroo Kanno Kazuya Akimitsu Junji Takabayashi |
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Affiliation: | National Institute of Agrobiological Sciences, NIAS, Tsukuba, Ibaraki 305-8602, Japan;, Program for Promotion of Basic Research Activities for Innovative Bioscience, Minato-ku, Tokyo 105-0001, Japan;, National Agricultural Research Center for Kyushu Okinawa Region, NARO, Koshi, Kumamoto 861-1192, Japan;, Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan;, and Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan |
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Abstract: | A pre-infestation of the white-backed planthopper (WBPH), Sogatella furcifera Horváth, conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae ( Xoo ) in rice ( Oryza sativa L.) under both laboratory and field conditions. The infestation of another planthopper species, the brown planthopper (BPH) Nilaparvata lugens Stål, did not significantly reduce the incidence of bacterial blight symptoms. A large-scale screening using a rice DNA microarray and quantitative RT-PCR revealed that WBPH infestation caused the upregulation of more defence-related genes than did BPH infestation. Hydroperoxide lyase 2 ( OsHPL2 ), an enzyme for producing C6 volatiles, was upregulated by WBPH infestation, but not by BPH infestation. One C6 volatile, ( E )-2-hexenal, accumulated in rice after WBPH infestation, but not after BPH infestation. A direct application of ( E )-2-hexenal to a liquid culture of Xoo inhibited the growth of the bacterium. Furthermore, a vapour treatment of rice plants with ( E )-2-hexenal induced resistance to bacterial blight. OsHPL2 -overexpressing transgenic rice plants exhibited increased resistance to bacterial blight. Based on these data, we conclude that OsHPL2 and its derived ( E )-2-hexenal play some role in WBPH-induced resistance in rice. |
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Keywords: | induced resistance hydroperoxide lyase (E)-2-hexenal (2E,6Z)-nonadienal C6 volatiles |
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