Highly frequent detection of transforming genes in acute leukemias by transfection using in vivo selection assays |
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| Authors: | H Hirai J Nishida F Takaku |
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| Affiliation: | 1. Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA;2. Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis School of Medicine, St. Louis, MO, USA;3. Molecular Cell Biology (MCB) graduate program, Division of Biology & Biomedical Sciences, Washington University in St. Louis School of Medicine, St. Louis, MO, USA;4. Department of Cellular Biology, University of Georgia, Athens, GA, USA;5. Department of Genetics, Washington University in St. Louis, St Louis, MO, USA;1. Department of Molecular Oncology & Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands;2. Oncode Institute, Utrecht, the Netherlands;3. Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam 1066 CX, the Netherlands |
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| Abstract: | DNAs from nine out of ten acute leukemia cases that were negative by in vitro focus forming assays exhibited transforming activity tested by in vivo selection assays in nude mice using transfected NIH3T3 cells. Of the nine cases, six cases contained activated N-ras genes, and one case exhibited activation of the c-K-ras gene. None of the ras gene family showed homology with the transforming genes derived from the other two cases. Our observations indicate that in vivo selection assays detect transforming genes including ras oncogenes at high frequency, and that activated N-ras genes are frequently detected in human acute leukemias. |
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