Survey of Mariner-Like Elements in the Housefly, Musca Domestica |
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| Authors: | M Yoshiyama H Honda T Shono K Kimura |
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| Institution: | (1) Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba, Ibaraki, 305-8572, Japan;(2) Laboratory of Apiculture, Department of Animal Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, P.O. Box 5, Tsukuba, Ibaraki, 3005-0901, Japan;(3) Laboratory of Apiculture, Department of Animal Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, P.O. Box 5, Tsukuba, Ibaraki, 305-0901, Japan |
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| Abstract: | The presence of mariner-like elements in four strains of the housefly, Musca domestica, was surveyed by PCR. Using the inverted terminal repeat (ITR) sequences of the Mos 1element as primers, DNAs were successfully amplified from all strains of the housefly. Southern blot analysis indicated that these amplified DNAs were repetitive sequences in the genome of M. domestica. Sequence analyses of cloned PCR products showed that they were 45% identical to the Mos 1element. These fragments appeared to be nonfunctional, because they contained no intact open reading frame (ORF) capable of encoding transposase. We conclude that these DNAs are degraded mariner-like elements (MLEs) in M. domestica. Because these endogenous MLEs in M. domesticado not encode any functional proteins, they probably would not affect the behavior of mariner-based vectors if such were introduced into this species as transformation vectors. |
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| Keywords: | mariner-like element Musca domestica transposable element |
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