Molecular cloning and nucleotide sequence cDNA encoding nucleoside diphosphate kinase of rice (Oryza sativa L.) |
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Authors: | Akira Yano Tetsuo Shimazaki Atsushi Kato Masaaki Umeda Hirofumi Uchimiya |
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Affiliation: | (1) Laboratory of Cellular Functions, Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Botany, Hokkaido University, 060 Sapporo, Japan |
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Abstract: | We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK gene is present in rice genome. |
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Keywords: | rice (Oryza sativa L.) nucleoside diphosphate kinase awd nm23 |
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