Replication kinetics of X chromosomes in fibroblasts and lymphocytes |
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Authors: | K S Reddy J R K Savage D G Papworth |
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Institution: | (1) MRC Radiobiology Unit, Chilton, OX11 ORD Didcot, UK;(2) Present address: Division of Human Genetics, St. John's Medical College, 560034 Bangalore, India;(3) B213, The Johns Hopkins Hospital, Baltimore, Maryland, USA |
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Abstract: | Summary The kinetics of replication for early and late replicating X chromosomes in karyotypically normal fibroblasts and lymphocytes was studied using terminal bromodeoxyuridine (BrdU) treatment followed by Hoechst/light/Giemsa staining. Although the order of band appearance differs between the two tissues, the programme (order and interval between band appearances) for early replicating bands (dark R-bands) is identical in the two homologues. This is probably also the case for later replicating bands (dark G-bands) though the criteria for derermining mean band appearance times are less reliable for these bands when terminal BrdU treatment is used. This means that the late X has a delayed start but thereafter proceeds at the same pace as its early counterpart. |
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