The role of calcium in neutrophil migration: the effect of calcium and calcium-antagonists in electroporated neutrophils. |
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| Authors: | J G Elferink G J Boonen B M de Koster |
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| Institution: | Department of Medical Biochemistry, University of Leiden, The Netherlands. |
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| Abstract: | Chemotaxis by electroporated rabbit peritoneal neutrophils in the absence of Ca2+ is only slightly different from that in the presence of Ca2+. Pretreatment of neutrophils with quin2-AM causes inhibition of chemotaxis. Calcium antagonists as nitrendipine and verapamil are inhibitory in nanomolar concentrations, while 10(5) times higher concentrations are required for inhibition of chemotaxis by neutrophils which were not electroporated. The results support the hypothesis that Ca2+ from Ca(2+)-storing organelles is of importance for chemotaxis, but that chemotaxis is not dependent on changes in cytoplasmic Ca2+ concentrations. |
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