Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells |
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| Authors: | N M Hoosein L Thim K H J?rgensen M G Brattain |
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| Institution: | Bristol-Baylor Laboratory, Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030. |
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| Abstract: | The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of 3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in 3H]thymidine incorporation of 50-60% occurred at 3-300 microM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at greater than 1 microM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced 3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties. |
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