Orientation and motion of a fluorescent probe in model membranes |
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| Authors: | R A Badley H Schneider W G Martin |
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| Affiliation: | 1. Key Laboratory of Materials for Energy Conversion, Chinese Academy of Sciences, Department of Materials Science and Engineering, University of Science and Technology of China, No. 96 Jinzhai Road, Hefei, Anhui Province 230026, China;2. Beijing National Laboratory for Condensed Matter Physics, The Institute of Physics, Chinese Academy of Sciences, No.8, 3rd South Street, Zhongguancun, Haidian, Beijing 100190, China;1. Institute of Nano Science and Nano Technology, University of Kashan, P.O. Box 87317-51167, Kashan, Islamic Republic of Iran;2. Department of Hematology and Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran;3. Department of Chemistry, College of Science, University of Raparin, Rania, Kurdistan Region, Iraq;4. Department of Chemistry, College of Science, International University of Erbil, Iraq;5. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Science, Kerman, Iran;1. Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P. O. Box. 87317–51167, Islamic Republic of Iran;2. Department of Chemistry, Kosar University of Bojnord, Bojnord, Islamic Republic of Iran;3. Young Researchers and Elites Club, Arak Branch, Islamic Azad University, Arak, Islamic Republic of Iran |
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| Abstract: | A method has been developed, based on polarized fluorescence intensity measurements, for studying the orientation and motion of fluorescent probes incorporated into lipid bilayers. Its use is illustrated in a study of the effect of cholesterol in egg lecithin multibilayers. When no cholesterol is present the probes are constrained to angles between 0° and 80° to the normal to the plane and show, superimposed, a rapid but restricted motion. The angular distribution decreases to a limiting range of 0° to 67° as cholesterol is increased to 25–30 mole % but the rapid motion is unaffected. |
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