Identification of potential targets in Staphylococcus aureus N315 using computer aided protein data analysis

Staphylococcus aureus is a gram positive bacterium, responsible for both community-acquired and hospital-acquired infection,resulting in a mortality rate of 39%. 43.2% resistance to methicilin and emerging resistance to Fluroquinolone and Oxazolidinone,have evoked the necessity of the establishment of alternative and effective therapeutic approach to treat this bacteria. In thiscomputational study, various database and online software are used to determine some specific targets of Staphylococcus aureusN315 other than those used by Penicillin, Quinolone and Oxazolidinone. For this purpose, among 302 essential proteins, 101 nonhomologousproteins were accrued and 64 proteins which are unique in several metabolic pathways of S. aureus were isolated byusing metabolic pathway analysis tools. Furthermore, 7 essentially unique enzymes involved in exclusive metabolic pathways wererevealed by this research, which can be potential drug target. Along with these important enzymes, 15 non-homologous proteinslocated on membrane were identified, which can play a vital role as potential therapeutic targets for the future researchers.