Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles |
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Authors: | Liesje Sintubin Wim De Windt Jan Dick Jan Mast David van der Ha Willy Verstraete Nico Boon |
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Institution: | (1) Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Gent, Belgium;(2) Avecom, Industrieweg 122P, 9032 Wondelgem, Belgium;(3) EM-unit of CODA-CERVA, Groeselenberg 99, 1180 Brussel, Belgium; |
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Abstract: | There is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. The use of
metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling
difficulties . Additionally, there exists a need for the development of a more eco-friendly production of nanosilver. In this
study, Gram-positive and Gram-negative bacteria were utilized in the non-enzymatic production of silver nanoparticles via
the interaction of silver ions and organic compounds present on the bacterial cell. Only lactic acid bacteria, Lactobacillus spp., Pediococcus pentosaceus, Enterococcus faecium, and Lactococcus garvieae, were able to reduce silver. The nanoparticles of the five best producing Lactobacillus spp. were examined more into detail with transmission electron microscopy. Particle localization inside the cell, the mean
particle size, and size distribution were species dependent, with Lactobacillus fermentum having the smallest mean particle size of 11.2 nm, the most narrow size distribution, and most nanoparticles associated with
the outside of the cells. Furthermore, influence of pH on the reduction process was investigated. With increasing pH, silver
recovery increased as well as the reduction rate as indicated by UV–VIS analyses. This study demonstrated that Lactobacillus spp. can be used for a rapid and efficient production of silver nanoparticles. |
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