Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection |
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Authors: | Larra W Yuelling Fang Du Peng Li Renata E Muradimova Zeng-jie Yang |
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Institution: | 1.Cancer Biology Program, Fox Chase Cancer Center, Temple University Health System |
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Abstract: | Microdissection is a novel technique that can isolate specific regions of a tissue and eliminate contamination from cellular sources in adjacent areas. This method was first utilized in the study of Nestin-expressing progenitors (NEPs), a newly identified population of cells in the cerebellar external germinal layer (EGL). Using microdissection in combination with fluorescent-activated cell sorting (FACS), a pure population of NEPs was collected separately from conventional granule neuron precursors in the EGL and from other contaminating Nestin-expressing cells in the cerebellum. Without microdissection, functional analyses of NEPs would not have been possible with the current methods available, such as Percoll gradient centrifugation and laser capture microdissection. This technique can also be applied for use with various tissues that contain either recognizable regions or fluorescently-labeled cells. Most importantly, a major advantage of this microdissection technique is that isolated cells are living and can be cultured for further experimentation, which is currently not possible with other described methods. |
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Keywords: | Neuroscience Issue 91 microdissection cerebellum EGL Nestin medulloblastoma |
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