首页 | 本学科首页   官方微博 | 高级检索  
     


Targeted resequencing of GWAS loci reveals novel genetic variants for milk production traits
Authors:Li Jiang  Xuan Liu  Jie Yang  Haifei Wang  Jicai Jiang  Lili Liu  Sang He  Xiangdong Ding  Jianfeng Liu  Qin Zhang
Affiliation:National Engineering Laboratory for Animal Breeding; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China; College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China
Abstract:

Background

Genome wide association study (GWAS) has been proven to be a powerful tool for detecting genomic variants associated with complex traits. However, the specific genes and causal variants underlying these traits remain unclear.

Results

Here, we used target-enrichment strategy coupled with next generation sequencing technique to study target regions which were found to be associated with milk production traits in dairy cattle in our previous GWAS. Among the large amount of novel variants detected by targeted resequencing, we selected 200 SNPs for further association study in a population consisting of 2634 cows. Sixty six SNPs distributed in 53 genes were identified to be associated significantly with on milk production traits. Of the 53 genes, 26 were consistent with our previous GWAS results. We further chose 20 significant genes to analyze their mRNA expression in different tissues of lactating cows, of which 15 were specificly highly expressed in mammary gland.

Conclusions

Our study illustrates the potential for identifying causal mutations for milk production traits using target-enrichment resequencing and extends the results of GWAS by discovering new and potentially functional mutations.

Electronic supplementary material

The online version of this article (doi:10.1186/1471-2164-15-1105) contains supplementary material, which is available to authorized users.
Keywords:Genetic variants   Milk production traits   Targeted resequencing
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号