Changes in activity of metabolic and regulatory pathways during germination of S. coelicolor |
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Authors: | Jan Bobek Eva Strakova Alice Zikova Jiri Vohradsky |
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Institution: | .Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Bioinformatics, Vídeňská 1083, 142 20 Prague 4, Czech Republic ;.Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Studničkova 7, 128 00 Praha 2, Czech Republic ;.Chemistry Department, Faculty of Science, J. E. Purkinje University, 400 96 Ústí nad Labem, Czech Republic |
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Abstract: | BackgroundBacterial spore germination is a developmental process during which all required metabolic pathways are restored to transfer cells from their dormant state into vegetative growth. Streptomyces are soil dwelling filamentous bacteria with complex life cycle, studied mostly for they ability to synthesize secondary metabolites including antibiotics.ResultsHere, we present a systematic approach that analyzes gene expression data obtained from 13 time points taken over 5.5 h of Streptomyces germination. Genes whose expression was significantly enhanced/diminished during the time-course were identified, and classified to metabolic and regulatory pathways. The classification into metabolic pathways revealed timing of the activation of specific pathways during the course of germination. The analysis also identified remarkable changes in the expression of specific sigma factors over the course of germination. Based on our knowledge of the targets of these factors, we speculate on their possible roles during germination. Among the factors whose expression was enhanced during the initial part of germination, SigE is though to manage cell wall reconstruction, SigR controls protein re-aggregation, and others (SigH, SigB, SigI, SigJ) control osmotic and oxidative stress responses.ConclusionsFrom the results, we conclude that most of the metabolic pathway mRNAs required for the initial phases of germination were synthesized during the sporulation process and stably conserved in the spore. After rehydration in growth medium, the stored mRNAs are being degraded and resynthesized during first hour. From the analysis of sigma factors we conclude that conditions favoring germination evoke stress-like cell responses.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-1173) contains supplementary material, which is available to authorized users. |
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Keywords: | Germination Streptomyces Microarrays Sigma factors Metabolic pathways |
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