Isolation,Cryopreservation and Culture of Human Amnion Epithelial Cells for
Clinical Applications |
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Authors: | Sean V Murphy Amritha Kidyoor Tanya Reid Anthony Atala Euan M Wallace Rebecca Lim |
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Institution: | 1.Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences;2.The Ritchie Centre, Monash Institute of Medical Research, Monash University |
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Abstract: | Human amnion epithelial cells (hAECs) derived from term or pre-term amnion membranes have
attracted attention from researchers and clinicians as a potential source of cells for
regenerative medicine. The reason for this interest is evidence that these cells have
highly multipotent differentiation ability, low immunogenicity, and anti-inflammatory
functions. These properties have prompted researchers to investigate the potential of
hAECs to be used to treat a variety of diseases and disorders in pre-clinical animal
studies with much success.hAECs have found widespread application for the treatment of a range of diseases and
disorders. Potential clinical applications of hAECs include the treatment of stroke,
multiple sclerosis, liver disease, diabetes and chronic and acute lung diseases.
Progressing from pre-clinical animal studies into clinical trials requires a higher
standard of quality control and safety for cell therapy products. For safety and quality
control considerations, it is preferred that cell isolation protocols use animal
product-free reagents. We have developed protocols to allow researchers to isolate, cryopreserve and culture
hAECs using animal product-free reagents. The advantage of this method is that these cells
can be isolated, characterized, cryopreserved and cultured without the risk of delivering
potentially harmful animal pathogens to humans, while maintaining suitable cell yields,
viabilities and growth potential. For researchers moving from pre-clinical animal studies
to clinical trials, these methodologies will greatly accelerate regulatory approval,
decrease risks and improve the quality of their therapeutic cell population. |
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Keywords: | Medicine Issue 94 Amnion Membrane Amniotic Stem Cells Epithelial Cell Therapy Perinatal Placenta |
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