Epidermal Growth Factor in Synaptosomal Fractions of Mouse Cerebral Cortex |
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Authors: | J. Lakshmanan M. E. Weichsel Jr. D. A. Fisher |
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Affiliation: | Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, California, U.S.A. |
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Abstract: | Using a specific and sensitive epidermal growth factor radioimmunoassay (EGF-RIA) we measured EGF concentrations in whole brain, cerebral cortex, and cerebral cortical synaptosomal (pinched-off presynaptic nerve terminals) fractions of 26-day-old mouse brain. The relative EGF concentration in synaptosomal fractions was significantly greater than the growth factor concentrations in whole brain or cerebral cortex. Intracerebral injection, in an amount of EGF, several-fold greater than whole brain EGF content, did not appreciably increase synaptosomal EGF concentration, suggesting that no artifact was involved. The high synaptosomal EGF content suggests a neurotransmitter or a neuromodulator role for EGF in the CNS. |
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Keywords: | Epidermal growth factor Synaptosome Cerebral cortex Neurotransmitter Neuromodulator |
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