In Vitro Culture of Orobanche aegyptiaca |
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Authors: | BEN-HOD GRAHAM; LOSNER DALIA; JOEL DANIEL M; MAYER ALFRED M |
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Institution: | * Department of Botany, The Hebrew University of Jerusalem 91904, Jerusalem
Department of Weed Research, ARO, Regional Experimental Station 31999, Newe Ya'ar, Israel |
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Abstract: | Calli derived from seedlings of the parasitic plant Orobancheaegyptiaca were grown on enriched nutrient agar. Growth wasenhanced by the addition of 0.1 mg 11 NAA and 5 % coconutmilk. The calli developed two kinds of elongated protrusions:thin ones which resemble the young root radicle and thick oneswhich resemble the adventitious roots of Orobanche plants. Bothformed pad-like structures when touching the substrate. In liquidculture, some of the calli were observed to surround isolatedcultured tomato roots. Orobanche aegyptiaca Pers., in vitro culture, germination, coconut milk, root parasite |
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