Molecular Characterization of Cyst Nematode Species (Heterodera spp.) from the Mediterranean Basin using RFLPs and Sequences of ITS-rDNA |
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| Authors: | M. Madani N. Vovlas P. Castillo S. A. Subbotin M. Moens |
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| Affiliation: | Authors' addresses: Crop Protection Department, Agricultural Research Centre, Burg, Van Gansberghelaan 96, 9820 Merelbeke, Belgium;;Istituto per la Protezione delle Piante, Sezione di Bari: Nematologia Agraria, Consiglio Nazionale delle Ricerche (C.N.R.), Via G. Amendola 165/A, 70126 Bari, Italy;;Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Cientificas (C.S.I.C.), Apdo. 4084, 14080 Cordoba, Spain;;Institute of Parasitology of the Russian Academy of Sciences, Leninskii Prospect 33, Moscow 119071, Russia;;University of Gent, Laboratory for Agrozoology, Coupure 555, 9000 Gent, Belgium (correspondence to S. A. Subbotin. E-mail: ) |
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| Abstract: | Fifteen populations of cyst‐forming nematodes belonging to 11 known and one unidentified species collected in countries bordering the Mediterranean Sea were studied using polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and internal transcribed spacer (ITS)‐rDNA sequences. RFLP profiles generated by the restriction enzymes AluI, AvaI, Bsh1236I, HaeIII, Hin6I, MvaI, PstI and RsaI are presented for Heterodera carotae, H. ciceri, H. fici, H. filipjevi, H. goettingiana, H. hordecalis, H. humuli, H. mediterranea, H. ripae and H. schachtii. Molecular data support the first detection of H. filipjevi from wheat in Italy and H. ripae from nettle in Greece. A relative high level of sequence divergence between populations of H. hordecalis was observed. This suggests that two species might presently be grouped under this taxon. The phylogenetic relationship between the Mediterranean cyst‐forming nematode species is analysed based on the ITS‐rDNA sequences. |
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| Keywords: | Heterodera carotae Heterodera ciceri Heterodera fici Heterodera filipjevi Heterodera goettingiana Heterodera hordecalis Heterodera humuli Heterodera mediterranea Heterodera ripae Heterodera schachtii internal transcribed spacer region phylogenetic relationships molecular identification |
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