|
|||||
|
|
Purification of herpesvirus nucleocapsids by fluorocarbon extraction. |
|
| Authors: | P Sottong R Harris B Graham B Rupp C Bell T Conlon J Klecker |
| Abstract: | Purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with Freon 113. Fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of DNA. CsCl banded DNA represented 30--40% of the theoretical yield of the total nucleocapsid population. |
| Keywords: | |