Recombinant protein,AlnA, combined with transgenic alfalfa remediates polychlorinated biphenyl-contaminated soils: efficiency and rhizosphere microbial community response |
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| Authors: | Hejun Ren Yu Su Junxing Zhang Hongyu Pan Baoyu Chen Yan Wang |
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| Institution: | 1.Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources,Jilin University,Changchun,People’s Republic of China;2.College of Plant Sciences,Jilin University,Changchun,People’s Republic of China;3.Research Institute of Agricultural Resources and Environment, Key Laboratory of Plant Nutrition and Agro-Environment in Northeast Region,Jilin Academy of Agricultural Sciences, MOA,Changchun,People’s Republic of China |
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| Abstract: | ObjectiveTo investigate the remediation efficiency of polychlorinated biphenyl (PCB)-contaminated soils by the combination of a bioemulsifying protein, AlnA, and alfalfa expressing bphC.ResultThe combination of AlnA and transgenic alfalfa promoted PCB soil remediation through the pot experiments. The removal rates of tri-PCBs (PCB 16/PCB 32 and PCB 31/PCB 28) and tetra-PCB (PCB 49) in transgenic alfalfa/AlnA treatment were 3.6-, 1.1-, and 2-fold higher than in transgenic alfalfa treatment alone. Analysis of gene copy number revealed that the PCB-degrading gene, bphC, of Pseudomonas-like bacterial populations in transgenic alfalfa/AlnA treatment increased 1.5-fold compared with that of unplanted soils. Bacterial community Illumina sequencing showed Pseudomonas, Arthrobacter, and Sphingomonas positively correlated with the removal rates of PCBs.ConclusionsPCB removal was unrelated to bacterial community diversity but positively correlated with their specific degraders and bphC gene expression. |
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