ObjectivesTo develop and validate a microdilution method for measuring the minimum inhibitory concentration (MIC) of biosurfactants.ResultsA standardized microdilution method including resazurin dye has been developed for measuring the MIC of biosurfactants and its validity was established through the replication of tetracycline and gentamicin MIC determination with standard bacterial strains.ConclusionThis new method allows the generation of accurate MIC measurements, whilst overcoming critical issues related to colour and solubility which may interfere with growth measurements for many types of biosurfactant extracts. |