Levansucrase from <Emphasis Type="Italic">Leuconostoc mesenteroides</Emphasis> NTM048 produces a levan exopolysaccharide with immunomodulating activity期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity

Authors:	Ryuichi Ishida Kouta Sakaguchi Chiaki Matsuzaki Toshihiko Katoh Nobuaki Ishida Kenji Yamamoto Keiko Hisa

Affiliation:	1.Research Institute for Bioresources and Biotechnology,Ishikawa Prefectural University,Nonoichi,Japan;2.Nitto Pharmaceutical Industries, Ltd.,Kyoto,Japan

Abstract:	Objectives A levansucrase from Leuconostoc mesenteroides NTM048 was cloned and expressed and its enzymatic product was characterized. Results The fructansucrase gene from Leuconostoc mesenteroides was cloned and expressed in Escherichia coli. The recombinant enzyme was purified as a single protein and its properties investigated. The polymer produced by the recombinant enzyme was identified as levan by various means including TLC and NMRs, and the enzyme was identified as a GH68 levansucrase. The enzyme was optimal at pH 5.5–6 and 30 °C, and its activity was stimulated by Ca²⁺. The levan produced by this strain induced IgA production in mice. Conclusion Leuconostoc mesenteroides, a probiotic strain, possessed levansucrase which catalyzed the produced levan that had immunomodulating activity.

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