不同药用植物根际土壤原核微生物多样性研究

为研究不同药用植物根际土壤中的原核微生物多样性，分别采集白术(Atractylodes macrocephala)、白芍(Paeonia sterniana)、牡丹(Paeonia suffruticosa)、玄参(Scrophularia ningpoensis)四种药用植物的根际土壤以及非种植区的土壤，针对16S rRNA基因的V3～V4区进行测序，分析土壤细菌群落的组成。结果表明，药用植物根际土壤中的细菌群落多样性指数显著高于非种植区土壤。五组样本的优势类群差异不大，总体相对丰度较高的有变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、芽单胞菌门(Gemmatimonadetes)、绿弯菌门(Chloroflexi)等，药用植物根际中的放线菌相对丰度高于非种植区。属水平上四种药用植物根际细菌和非种植区的群落结构有较大差异，四种中药材的根际土壤中各自富集了特异性的有益细菌属。药用植物根际土壤中的NMD1、Dongia、Gaiella、Streptomyces等相对丰度高于非种植区，而非种植区土壤中Lysoba...

Diversity of Prokaryotic Microorganisms in Rhizosphere Soil of Different Medicinal Plants

In order to study the microbial diversity in rhizospheric soil of different medicinal plants, the rhizospheric soil of Atractylodes macrocephala, Paeonia sterniana, Paeonia suffruticosa, Scrophularia ningpoensis as well as soil of non-planting area were collected respectively. The V3-V4 region of 16S rRNA gene was targeted and sequenced to analyze the composition of soil microbial community. The results showed that the bacteria diversity index in rhizosphere of medicinal plants was significantly higher than that in non-planting soil. The dominant groups of five groups of samples are not significantly different, and the overall relative abundances of Proteobacteria, Anhydrideobacteria, Actinobacteria, Gemmatimonadetes, and Chloroflexi are relatively high. The relative abundance of Actinomycetes in the rhizosphere of medicinal plants was obviously higher than that in non-planted area. At the genus level, there are greater differences in the community structure between four medicinal plants and the non-planted groups. The rhizosphere soils of the four Chinese herbal medicines were enriched with specific genera of beneficial bacteria. The relative abundance of NMD1, Dongia, Gaiella, and Streptomyces in rhizosphere soil of medicinal plants was obviously higher than that in the non-planting area, and the relative abundance of Lysobacter in soil of the non-planted area was significantly higher than that of medicinal plants. In addition, the functions of nitrate reduction and aromatic compound degradation were enriched in the rhizosphere soil of the four Chinese medicinal materials.