The hepatocyte calcium oscillator |
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Authors: | P H Cobbold A Sanchez-Bueno C J Dixon |
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Institution: | Department of Human Anatomy & Cell Biology, University of Liverpool, UK. |
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Abstract: | Hepatocytes stimulated with calcium-mobilising agonists generate free Ca transients whose frequency is modulated by hormone concentration. Importantly, the time-course of individual free Ca transients is independent of agonist dose but does change with agonist species. A receptor-controlled model in which protein kinase C provides negative feedback directed against the different receptors, or receptor-specific G proteins, has been proposed in order to explain the agonist-specificity of the falling phase of the free Ca spikes. Here we show further evidence, from mixing of hormones and from the effects of elevated cAMP, of receptor-specific information within the spikes. |
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