Preparative isolation of hydroxylysine and its O-glycosides from human urine |
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| Authors: | R T Markiw |
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| Affiliation: | 1. Kuban State University, Krasnodar 350040, Russia;2. CanAm Bioresearch Inc., Winnipeg, MB R3T 0P4, Canada;3. School of Bioengineering and Health, Wuhan Textile University, China;1. Fukuoka Prefectural Institute of Health and Environmental Sciences, 39 Mukaisano, Dazaifu, Fukuoka 818-0135, Japan;2. The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu City, Fukuoka 808-0135, Japan |
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| Abstract: | Amino acids were conveniently isolated from urine by a combination of weak anion- and strong cation-exchange resins and fractionated into groups by displacement chromatography. Glucosylgalactosylhydroxylysine and galactosylhydroxylysine were subsequently separated by paper chromatography, whereas hydroxylysine and lysine were separated by ion-exchange chromatography. |
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