Increase in plasmid transformation efficiency in SOS-induced Escherichia coli cells |
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| Authors: | J.-Albert Vericat, Ricardo Guerrero Jordi Barbé |
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| Affiliation: | (1) Department of Genetics and Microbiology, Autonomous University of Barcelona, Bellaterra, E-08193 Barcelona, Spain;(2) Present address: Frederick Cancer Research Facility, National Cancer Institute, P.O. Box B, 21701 Frederick, MD, USA |
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| Abstract: | Summary UV irradiation of competent cells of Escherichia coli K12 produced an increase in the efficiency of transformation with plasmid DNA. This phenomenon has been called IPTE (increase in plasmid transformation efficiency) and is dependent on the activated state of the RecA protein. IPTE is independent of the lexA, recB recC, and recF genes. It is not related to the size or the replicon type of the plasmid. Furthermore, it is also induced in cells which have been previously treated with other SOS system-inducing agents such as bleomycin, mitomycin C, or nalidixic acid. IPTE is therefore similar to other repair (SOS) functions inducible by DNA damage since all of them are dependent upon activation of the RecA protein. IPTE differs from other SOS functions in the absence of a direct control by the LexA repressor. |
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| Keywords: | SOS repair Bacterial transformation Plasmid DNA UV radiation |
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