Cloning and regulation of the expression of the lysA gene from Bacillus subtilis]

Cloning of Bacillus subtilis DNA fragment with the lysA gene encoding diaminopimelatecarboxylase (EC 4.1.1.20) was done. The cloned gene in poorly expressed both in Escherichia coli and in Bacillus subtilis. Some DNA sequence distant from the lysA gene seems to be necessary for full gene expression, this sequence having been not cloned together with the lysA. The sequence in needed for regulation of the expression as well.