The three-dimensional arrangement of intermediate filaments in Romney wool cortical cells |
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Authors: | Caldwell Jonathan P Mastronarde David N Woods Joy L Bryson Warren G |
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Affiliation: | Canesis Network Ltd (formerly Wool Research Organisation of New Zealand Inc.), Private Bag 4749, Christchurch, New Zealand. jonathan.caldwell@canesis.com |
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Abstract: | The three-dimensional orientation and arrangement of intermediate filaments in Romney wool ortho-, meso-, and paracortical cells has been revealed using single axis high voltage electron tomography. Modelled tomograms confirm that intermediate filaments in orthocortical cells are arranged helically, with the helical angle progressively increasing from the centre to the periphery of macrofibrils. Intermediate filaments in meso- and paracortical cells display parallel arrangements differing mainly in packing density, with the mesocortex packed more tightly than the paracortex. The intermediate filament arrangements observed confirm expectations based on earlier two-dimensional transmission electron microscopy observations by the authors and other researchers. It is expected that these findings will contribute to a better understanding of the biological and structural basis of wool fibre curvature. |
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Keywords: | Cortical cell Staple crimp Fibre curvature High voltage electron microscopy Intermediate filaments Mesocortex Orthocortex Paracortex Romney Three-dimensional Tomography Wool |
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