RFLP and semi-multiplex PCR-based identification of four eel species from the south-western Indian Ocean region |
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| Authors: | P. A. Gagnaire,¶ ,K. Tsukamoto,&dagger ,J. Aoyama,&dagger ,Y. Minegishi,&dagger ,P. Valade,&Dagger P. Berrebi |
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| Affiliation: | Institut des Sciences de l'Evolution, UMR 5554, UniversitéMontpellier 2, cc065, place E. Bataillon, 34095 Montpellier cedex 05, France; , Ocean Research institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164-8639, Japan; and Association Réunionnaise de Développement de l'Aquaculture, Z.I. Les Sables, B.P. 16, 97427 Etang-Salé, Ile de La Réunion, France |
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| Abstract: | To make the assignment of elvers to species more easily attainable and reliable, unpublished and recently published mitochondrial DNA sequences were used to design molecular determination approaches not based on sequencing. Two methods were proposed based on 16SrRNA single nucleotide polymorphism analysis, which improve identification of the four eel species living in the south-western Indian Ocean region. |
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| Keywords: | Anguilla mtDNA polymorphism RFLP semi-multiplex PCR species identification |
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