| 豌豆肌动蛋白异型体基因的特异性表达 |
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| 引用本文： | 凌毅,赵武玲. 豌豆肌动蛋白异型体基因的特异性表达[J]. 植物学通报, 2001, 18(1): 76-80 |
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| 作者姓名： | 凌毅 赵武玲 |
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| 作者单位： | 1. 中国农业大学西校区图书馆,
2. 中国农业大学农业部 |
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| 基金项目： | 国家自然科学基金资助项目（39870077）。Supported by the National Natural Science Founation of China (No. 39870077). |
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| 摘 要： | 将分属三类豌豆（Pisum sativum）卷须肌动蛋白异型体（PEAc）的基因3'端进行了亚克隆，并利用限制性内切酶从中切聚了完整的3'非翻译区片段，将之做为特异性探针进行DIG标记，并对不同发育时间豌豆根、茎、叶及花粉中提取的总RNA进行点杂交分析。发现其中Ⅰ类（PEAcⅠ）和Ⅱ类（PEAcⅡ）在根、茎、叶中都有表达，但存在发育时间上的表达物质特异性，尤其中叶叶的差异较明显，Ⅲ类（PEAcⅢ）与前二者的差别更大，仅发现其有2d及28d的根、7d的茎及18d的叶中有表达。在花粉中，只有PEAcⅡ有少量的表达，说明在类异型体基因的表达还存在组织特异性。
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| 关 键 词： | 肌动蛋白基因 异型体 3‘非翻译区 特异性表达 豌豆 |
| 修稿时间： | 2000-03-21 |
Specific Expression of PeaIsoactin Genes |
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| LING Yi,ZHAO Wu-Ling. Specific Expression of PeaIsoactin Genes[J]. Chinese Bulletin of Botany, 2001, 18(1): 76-80 |
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| Authors: | LING Yi ZHAO Wu-Ling |
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| Affiliation: | LING Yi 2ZHAO Wu_Ling [WT6BZ] 1 |
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| Abstract: | The authors subcloned 3' termination of three isoactin genes of Pea ( Pisum sativum ) tendril (PEAc) into pBS vector. Then, the entire 3'_UTRs of these three PEAc were obtained using restriction endonuclease cleavage. Using DIG labeled 3'_UTR fragments as specific_probes, RNA Dot blot showed that PEAcI and PEAcII expressed in roots, stems and leave, and could express at different development stage, especially in leave. Expression of PEAcIII were different from the other two, its mRNA could only be detected in 2nd day and 28th day root, 7th day stem and 18th day leaf. In pollen grains, only PEAcII could be detected. |
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| Keywords: | Actin gene Isoform 3'_untranslated region (3'_UTR) Specific expression |
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