Search for genes influencing the maintenance of the [ISP+] prion-like antisuppressor determinant in yeast with the use of an insertion gene library |
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Authors: | T. M. Rogoza O. V. Viktorovskaya S. A. Rodionova M. S. Ivanov K. V. Volkov L. N. Mironova |
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Affiliation: | (1) Department of Genetics and Breeding, St. Petersburg State University, St. Petersburg, 199034, Russia |
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Abstract: | The prion-like determinant [ISP +] manifests itself as an antisuppressor of certain sup35 mutations. To establish that [ISP +] is indeed a new yeast prion, it is necessary to identify the gene that codes for the protein whose prion form is [ISP +]. Analysis of the transformants obtained by transformation of an [ISP +] strain with an insertion gene library revealed three genes controlling the [ISP +] maintenance: UPF1, UPF2, and SFP1. SFP1 codes for a potentially prionogenic protein, which is enriched in Asn and Gln residues, and is thereby the most likely candidate for the [ISP +] structural gene. UPF1 and UPF2 code for components of nonsense-mediated mRNA decay. The [ISP +] elimination caused by UPF1 and UPF2 inactivation was reversible, and Upf1p and Upf2p were not functionally related to phosphatase Ppz1p, which influences the [ISP +] manifestation. Possible mechanisms sustaining the influence of UPF1 and UPF2 on [ISP +] maintenance are discussed. |
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Keywords: | yeast nonsense suppression yeast prions insertion gene library UPF1 UPF2 SFP1 PPZ1 |
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