Comparison of the Ames assay and the induction of sister chromatid exchanges: Results with ten pharmaceuticals and five selected agents |
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| Authors: | Esmail K. Shubber David Jacobson-Kram Dr. Jerry R. Williams |
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| Affiliation: | (1) Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq;(2) Radiobiology Laboratory, The Johns Hopkins Oncology Center, Baltimore, Maryland;(3) Radiobiology Laboratory, The Johns HYopkins Oncology Center, 600 North Wolfe Street, Room 2-121, 21205 Baltimore, MD |
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| Abstract: | Seven antischistosomal drugs, two antimalarial drugs, and one antiamoebic drug were tested in all five Ames strains for induction of mutation, as well as for induction of cytotoxicity, inhibition of cellular progression, and the induction of sister chromatid exchanges in two cultured mammalian cell lines. We found that two agents shown to be negative in the Ames test were positive for sister chromatid exchange induction. Based on qualitative and quantitative evaluation, we find that all but three of the pharmaceuticals should be considered to be potential human carcinogens.Abbreviations AA 2-aminoanthracene - 9AACC 9-aminoacridine - AM amoscanate - BrdUU bromodeoxyuridine - CA chloroquine diphosphate - CHO Chinese hamster ovary - CQ chloroquine - DAPI 4 6-diamidino-2-phenylindole - DHY dehydroemetine - DMSO dimethyl sulfoxide - EB ethidium bromide - FCS fetal calf serum - FN 4-fluoro-3-nitrophenyl azide - HY hycanthone - ICP inhibiting cell progression - LU lucanthone - MEM minimal essential medium - 2NF 2-nitrofurantoin - 4NPD 4-nitro-O-phenylenediamine - NZ niridazole - OL oltipraz - OX oxaminiquine - PBS phosphate buffered saline - PQ primaquine - PZ praziquantel - SA sodium azide - SCE sister chromatid exchange |
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| Keywords: | Ames assay antiamoebic drug antimalarial drug antischistosomal drug mutagen screening |
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