HMGCoA reductase and cholesterol 7 alpha-hydroxylase in human liver |
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| Authors: | E Bosisio G Cighetti M Galli Kienle R Tritapepe G Galli |
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| Affiliation: | 1. Institute of Pharmacology and Pharmacognosy, University of Milan - Via A.del Sarto 21 - 20129 Milano, Italy;2. Department of Medical Chemistry and Biochemistry, University of Milan - Via Saldini 50 - 20133 Milano, Italy;3. 3rd Surgical Clinic, School of Medicine University of Milan - Via F. Sforza 35 - 20122 Milano, Italy |
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| Abstract: | The activities of HMGCoA reductase and cholesterol 7 alpha-hydroxylase were assayed in liver biopsies of patients with or without cholestyramine treatment. The active dephosphorylated form of HMGCoA reductase and the activity of cholesterol 7 alpha-hydroxylase were under the detection limits in untreated subjects. After cholestyramine treatment activities of both enzymes were stimulated and the active form of HMGCoA reductase became detectable in four out of the five tested patients. In two subjects who received cholestyramine, the effect of exogenously added [4-14C] cholesterol on cholesterol 7 alpha-hydroxylase was tested. In the presence of Tween 80, the detergent by which [14C]cholesterol was suspended, the enzyme activity was profoundly inhibited and synthesis of 7 alpha-hydroxycholesterol was extremely low. |
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