Oxygenation alters ganglioside expression in rat liver following partial hepatectomy |
| |
Authors: | Markoti? Anita Culi? Vedrana Cikes Kurir Tina Ticinovi? Meisen Iris Büntemeyer Heino Boraska Vesna Zemunik Tatijana Petri Nadan Mesari? Marko Peter-Katalini? Jasna Müthing Johannes |
| |
Institution: | Department of Biochemistry, Split University Medical School, Soltanska 2, 21000 Split, Croatia. markotic@bsb.mefst.hr |
| |
Abstract: | Gangliosides from livers of weanling rats were analyzed after 15% partial hepatectomy (PH) and different pre- and post-operative hyberbaric oxygenation (pre- and postHBO). Neu5Ac was the predominant ganglioside-derived sialic acid (>85%) compared to Neu5Gc. Almost identical low total sialic acid content (Neu5Ac+Neu5Gc) of the control and operated nonHBO animals opposed a 6.4- to 7.6-fold increase in pre- and postHBO animals (69.26 and 81.64pmol/mg wet weight, respectively). NanoESI-QTOF mass spectrometry combined with HPTLC immunostaining revealed GM3(Neu5Ac) and GM3(Neu5Gc) as major gangliosides, correlating with the respective sialic acid concentrations. Minor neolacto-series gangliosides were enhanced in preHBO and postHBO, but GM1-core gangliosides only in preHBO rats. GM2 and GalNAc-GM1b were clearly detectable in oxygenated rats compared to traces in the control and nonHBO animals. These results point at a functional role of gangliosides in liver growth regulation and reconstitution after PH combined with pre- and post-operative HBO treatment. |
| |
Keywords: | HPLC HPTLC Immunostaining NanoESI-QTOF mass spectrometry Sialic acid Partial hepatectomy Oxygenation Gangliosides |
本文献已被 ScienceDirect PubMed 等数据库收录! |