Mutagenic treatments result in inactivation of expression of a transfected bacterial gene integrated into a human cell line |
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Authors: | A R Lehmann C F Arlett S A Harcourt H Steingrimsdottir M M Gebara |
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Institution: | MRC Cell Mutation Unit, University of Sussex, Brighton, Great Britain. |
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Abstract: | The cell line E2 is a SV40-transformed human fibroblast cell line containing a single integrated copy of the bacterial guanine phosphoribosyl transferase (gpt) gene. Treatment of E2 with ultraviolet light (UV) or ethyl methanesulphonate (EMS) induced the formation of Gpt- derivatives. Several induced derivatives have been isolated, and the structure, expression and revertibility of the gpt gene have been analysed. In the majority of cases the Gpt- phenotype resulted from switching off the gpt gene, in most instances by methylation, but in a few cases by phenotypic switching. Thus mutagenic treatment can result in the inactivation of gene expression in human cells. In a small proportion of Gpt- derivatives the gpt sequences were deleted. |
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