Detection of IgG antibody to Bovine Leukaemia Virus in urine and serum by two enzyme immunoassays |
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| Authors: | K. T. Carli A. Sen H. Batmaz E. Kennerman |
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| Affiliation: | Department of Microbiology, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey. tayfun@uu20.bim.uludag.edu.tr |
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| Abstract: | Four hundred blood sera from a cattle production unit were tested for BLV-(Bovine Leukaemia Virus) antibody with IP (Institut Porquier) and SB (Svanova Biotech) ELISA kits. Seventy-seven cattle with BLV-antibody (19.25%) and 77 without the antibody were used. No significant difference was found between O.D. of sera of PL+ (Persistent Lymphocytosis Positive) and PL- (Negative) cattle. The mean O.D. of urine samples of 77 seropositive cattle was significantly higher than that of 77 seronegative cattle (P < 0.01). There were also differences between urine O.D.s of seropositive (PL+) and seropositive (PL-) groups of cattle with IP (P < 0.05) and SB (P < 0.01) kits. All the results revealed the presence of BLV-antibody in the urine of the cattle without any urinary dysfunction. |
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