Use of a perfusion technique for measurements of respiratory activity in cultured cells |
| |
Authors: | Anders Peterson Erik Walum |
| |
Institution: | 1. Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Enk?pingsv?gen 126, S-172 46, Sundbyberg, Sweden
|
| |
Abstract: | Summary A method for measuring respiratory activity in anchorage-dependent cultured cells has been developed. This method is based
on a technique that permits the perfusion of standard plastic culture dishes with attached cells. Basal respiratory activities
were studied in two continuous cell lines of neural origin, neuroblastoma C1300 clone 41A3 and glioma 138MG. As compared to traditional measurements on detached cells, a fourfold increase in value was obtained. Investigations
on membrane permeability suggested that the observed difference could be attributed to alterations in cell membrane integrity.
Pretreatment with dibutyryl cyclic AMP, known to induce a morphological and biochemical differentiation in C1300 and 138MG
cells, caused in both cell lines an enhanced respiration.
This work was supported by grants from the National Swedish Board for Technical Development (grant 82-5025). |
| |
Keywords: | neural cell lines respiratory activity perfusion polarographic measurements dibutyryl cyclic AMP |
本文献已被 SpringerLink 等数据库收录! |
|